Complete genome sequences of tolaasin-detoxifying Mycetocola species isolated from oyster mushroom

From the paper

Abstract

Complete genome sequences of tolaasins-detoxifying Mycetocola spp., M. lacteus MAFF 211326, M. saprophilus MAFF 211324, and M. tolaasinivorans MAFF 211325, were determined. The length of circular chromosome of these strains was 3.47, 3.52, and 3.49 Mbp, respectively, and a 28,409-bp plasmid was found in M. lacteus MAFF 211326. Genomic comparisons of mushroom-associated, -pathogenic, and environmental strains of Mycetocola spp. showed that the genomic synteny of chromosomes was largely shared among Mycetocola spp. Furthermore, the previously estimated catalytic triad of tolaasin-degrading factor (Tdf) is conserved among the genomes of strains isolated from mushrooms and non-mushroom substrates. Lipopeptides, including tolaasins, are biologically active and involved in various biological interactions. The antagonistic traits of Mycetocola spp. might not be restricted to tolaasin detoxification; therefore, genome information of Mycetocola spp. will facilitate the mining and identification of enzymes that cleave cyclic lipopeptides acting in various biological interactions observed in agricultural and ecological contexts.