Effect of the main ingredients of Rhizoma gastrodiae on mycelial biomass and exopolysaccharide productions by submerged culture of Grifola frondosa

From the paper

Abstract

Various concentrations of the main ingredients of Rhizoma gastrodiae including gastrodin (GA) and p‐hydroxybenzyl alcohol (HA) as well as p‐hydroxylbenzaldehyde (HBA) were separately added into the cultural medium of Grifola frondosa in submerged culture. The results showed that the additive concentration of gastrodin from 0.10 to 0.35 g L⁻¹, p‐hydroxybenzyl alcohol from 0.05 to 0.30 g L⁻¹ and p‐hydroxylbenzaldehyde from 0.05 to 0.30 g L⁻¹ can significantly promote the EPS production by submerged culture of G. frondosa (P < 0.05). Among them, the addition of p‐hydroxylbenzaldehyde at 0.15 g L⁻¹ exhibited the best results, and the EPS productions reached 2.2511 ± 0.0378 g L⁻¹, with an increase of 45.03% compared with the control (without additives). Furthermore, all of the additives had an effective stimulatory effect on mycelial biomass. In addition, there is an important and novel breakthrough that p‐hydroxylbenzaldehyde was more effective than R. gastrodiae extract on increasing the EPS productions.