Double-stranded RNA and virus-like particles in the edible basidiomycete Flammulina velutipes (Enokitake).

Flammulina velutipes

Energy & fatigueRespiratory

SpeciesEnoki, Winter Mushroom

JournalFEMS microbiology letters

Year1999

Key points

One of the commercial strains of Flammulina velutipes was analyzed for the presence of double-stranded RNA (dsRNA) elements to examine the underlying mechanism of strain degeneration
As a result, two dsRNA elements sized 1.9 and 1.8 kb were detected in mycelium derived from spontaneously brown-colored fruit body
They were not detected in the normal strains or in fruiting-impaired degenerative isolates
The dsRNAs were not in the nuclear or mitochondrial fractions, but were located in the cytoplasmic fraction
The presence of virus-like particles of ca. 50 nm diameter associated with the dsRNAs was confirmed by electron microscopic observation

From the paper

Abstract

One of the commercial strains of Flammulina velutipes was analyzed for the presence of double-stranded RNA (dsRNA) elements to examine the underlying mechanism of strain degeneration. As a result, two dsRNA elements sized 1.9 and 1.8 kb were detected in mycelium derived from spontaneously brown-colored fruit body. They were not detected in the normal strains or in fruiting-impaired degenerative isolates. The dsRNAs were not in the nuclear or mitochondrial fractions, but were located in the cytoplasmic fraction. The presence of virus-like particles of ca. 50 nm diameter associated with the dsRNAs was confirmed by electron microscopic observation.

Citation

Magae Y, Hayashi N (1999). Double-stranded RNA and virus-like particles in the edible basidiomycete Flammulina velutipes (Enokitake). FEMS microbiology letters https://doi.org/10.1111/j.1574-6968.1999.tb08814.x PMID: 10556730

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