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Enoki, Winter Mushroom · 2000 · Research Support, Non U.S. Gov'T

Medium relevance

Cloning and sequencing analysis of Trp1 gene of Flammulina velutipes.

Flammulina velutipes

SpeciesEnoki, Winter Mushroom
JournalFEMS microbiology letters
Year2000

Key points

  • The genomic TRP1 gene from basidiomycete Flammulina velutipes was cloned by complementation of yeast Saccharomyces cerevisiae trp1 mutation
  • Sequencing analysis revealed that the TRP1 gene encoded a single protein consisting of three catalytic functional domains; glutamine amidotransferase, indole-3-glycerol phosphate synthase ) and N-(5'-phosphoribosyl) anthranilate isomerase, in order of NH2-glutamine amidotransferase-indole-3-glycerol phosphate synthase N-(5'-phosphoribosyl) anthranilate isomerase-COOH. The coding sequence of the TRP1 gene was interrupted by a single intron of 48 bases, the position and flanking sequences of which were highly homologous to those of basidiomycete Phanerochaete chrysosporium trpC

From the paper

Abstract

The genomic TRP1 gene from basidiomycete Flammulina velutipes was cloned by complementation of yeast Saccharomyces cerevisiae trp1 mutation. Sequencing analysis revealed that the TRP1 gene encoded a single protein consisting of three catalytic functional domains; glutamine amidotransferase, indole-3-glycerol phosphate synthase ) and N-(5'-phosphoribosyl) anthranilate isomerase, in order of NH2-glutamine amidotransferase-indole-3-glycerol phosphate synthase N-(5'-phosphoribosyl) anthranilate isomerase-COOH. The coding sequence of the TRP1 gene was interrupted by a single intron of 48 bases, the position and flanking sequences of which were highly homologous to those of basidiomycete Phanerochaete chrysosporium trpC.

Citation

Nakai R, Sen K, Kurosawa S, Shibai H (2000). Cloning and sequencing analysis of Trp1 gene of Flammulina velutipes. FEMS microbiology letters https://doi.org/10.1111/j.1574-6968.2000.tb09261.x PMID: 10981689

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