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Enoki, Winter Mushroom · 2000 · Research Support, Non U.S. Gov'T

Medium relevance

Cloning and sequencing analysis of Trp1 gene of Flammulina velutipes.

Flammulina velutipes

Immune support
SpeciesEnoki, Winter Mushroom
JournalFEMS microbiology letters
Year2000

Key points

  • The genomic TRP1 gene from basidiomycete Flammulina velutipes was cloned by complementation of yeast Saccharomyces cerevisiae trp1 mutation
  • Sequencing analysis revealed that the TRP1 gene encoded a single protein consisting of three catalytic functional domains; glutamine amidotransferase, indole-3-glycerol phosphate synthase ) and N-(5'-phosphoribosyl) anthranilate isomerase, in order of NH2-glutamine amidotransferase-indole-3-glycerol phosphate synthase N-(5'-phosphoribosyl) anthranilate isomerase-COOH. The coding sequence of the TRP1 gene was interrupted by a single intron of 48 bases, the position and flanking sequences of which were highly homologous to those of basidiomycete Phanerochaete chrysosporium trpC

Metadata-grounded summary

Citation abstract

The genomic TRP1 gene from basidiomycete Flammulina velutipes was cloned by complementation of yeast Saccharomyces cerevisiae trp1 mutation. Sequencing analysis revealed that the TRP1 gene encoded a single protein consisting of three catalytic functional domains; glutamine amidotransferase, indole-3-glycerol phosphate synthase ) and N-(5'-phosphoribosyl) anthranilate isomerase, in order of NH2-glutamine amidotransferase-indole-3-glycerol phosphate synthase N-(5'-phosphoribosyl) anthranilate isomerase-COOH. The coding sequence of the TRP1 gene was interrupted by a single intron of 48 bases, the position and flanking sequences of which were highly homologous to those of basidiomycete Phanerochaete chrysosporium trpC.

Citation

Nakai R, Sen K, Kurosawa S, Shibai H (2000). Cloning and sequencing analysis of Trp1 gene of Flammulina velutipes. FEMS microbiology letters https://doi.org/10.1111/j.1574-6968.2000.tb09261.x PMID: 10981689

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