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King Oyster, Eryngii · 2000 · Research Support, Non U.S. Gov'T

High relevance

The cloning of a new peroxidase found in lignocellulose cultures of Pleurotus eryngii and sequence comparison with other fungal peroxidases.

Pleurotus eryngii

Gut & microbiome
SpeciesKing Oyster, Eryngii
JournalFEMS microbiology letters
Year2000

Key points

  • We report cloning and sequencing of gene ps1 encoding a versatile peroxidase combining catalytic properties of lignin peroxidase (LiP) and manganese peroxidase (MnP) isolated from lignocellulose cultures of the white-rot fungus Pleurotus eryngii
  • The gene contains 15 putative introns, and the deduced amino acid sequence consists of a 339-residue mature protein with a 31-residue signal peptide
  • Several putative response elements were identified in the promoter region
  • Amino acid residues involved in oxidation of Mn(2+) and aromatic substrates by direct electron transfer to heme and long-range electron transfer from superficial residues as predicted by analogy with Phanerochaete chrysosporium MnP and LiP, respectively
  • A dendrogram is presented illustrating sequence relationships between 29 fungal peroxidases

Metadata-grounded summary

Citation abstract

We report cloning and sequencing of gene ps1 encoding a versatile peroxidase combining catalytic properties of lignin peroxidase (LiP) and manganese peroxidase (MnP) isolated from lignocellulose cultures of the white-rot fungus Pleurotus eryngii. The gene contains 15 putative introns, and the deduced amino acid sequence consists of a 339-residue mature protein with a 31-residue signal peptide. Several putative response elements were identified in the promoter region. Amino acid residues involved in oxidation of Mn(2+) and aromatic substrates by direct electron transfer to heme and long-range electron transfer from superficial residues as predicted by analogy with Phanerochaete chrysosporium MnP and LiP, respectively. A dendrogram is presented illustrating sequence relationships between 29 fungal peroxidases.

Citation

Camarero S, Ruiz-Dueñas FJ, Sarkar S, Martínez MJ, Martínez AT (2000). The cloning of a new peroxidase found in lignocellulose cultures of Pleurotus eryngii and sequence comparison with other fungal peroxidases. FEMS microbiology letters https://doi.org/10.1111/j.1574-6968.2000.tb09316.x PMID: 11004397

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