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Enoki, Winter Mushroom · 2006 · Research Support, Non U.S. Gov'T

Medium relevance

Isolation and analysis of genes specifically expressed during fruiting body development in the basidiomycete Flammulina velutipes by fluorescence differential display.

Flammulina velutipes

Immune support
SpeciesEnoki, Winter Mushroom
JournalFEMS microbiology letters
Year2006

Key points

  • Using fluorescence differential display, cDNAs specifically expressed at the primordial stage of fruiting body development were isolated from the basidiomycete, Flammulina velutipes
  • Seventy-five cDNAs were sequenced and compared with the amino-acid sequences of proteins in the database by BLASTX search
  • Significant similarity was found for 29 cDNAs coding for proteins with known function, GTP-binding protein, growth factor, ubiquitin-proteasome, cytochrome P450 and hydrophobin, all of which would be associated with fruiting body development
  • Seventeen cDNAs were not similar to proteins in the database and may represent unique genes that play specific roles in the process of fruiting in F. velutipes

Metadata-grounded summary

Citation abstract

Using fluorescence differential display, cDNAs specifically expressed at the primordial stage of fruiting body development were isolated from the basidiomycete, Flammulina velutipes. Seventy-five cDNAs were sequenced and compared with the amino-acid sequences of proteins in the database by BLASTX search. Significant similarity was found for 29 cDNAs coding for proteins with known function, GTP-binding protein, growth factor, ubiquitin-proteasome, cytochrome P450 and hydrophobin, all of which would be associated with fruiting body development. Seventeen cDNAs were not similar to proteins in the database and may represent unique genes that play specific roles in the process of fruiting in F. velutipes.

Citation

Yamada M, Sakuraba S, Shibata K, Taguchi G, Inatomi S, Okazaki M, et al. (2006). Isolation and analysis of genes specifically expressed during fruiting body development in the basidiomycete Flammulina velutipes by fluorescence differential display. FEMS microbiology letters https://doi.org/10.1111/j.1574-6968.2005.00023.x PMID: 16451195

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