The NF- κB Signaling and Wnt/ β-catenin Signaling in MCF-7 Breast Cancer Cells in Response to Bioactive Components from Mushroom Antrodia Camphorata.

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Citation abstract

Breast cancer is the leading cancer, accounting for approximately 15% cancer deaths in women worldwide. This study investigated the anti-inflammation and anticancer properties of two bioactive components from Antrodia camphorata ( AC ), a rare medicinal mushroom natively grown in Taiwan and commonly used in Chinese traditional medicine. The anti-inflammatory and antitumorigenic functions of Antroquinonol (AQ) and 4-Acetylantroquinonol B (4-AAQB) from AC were examined on breast cancer cell line MCF-7 with/without TNF- α stimulation. Among nine inflammatory mediators ( IL6, IL10, IL1 β, IFN γ, PTGS2, TGF β 1, TNF- α, CCL2 and CSF1 ) examined, AQ inhibited two of them ( IL-10 and PTGS2 ), while 4-AAQB inhibited three of them ( IL-10, PTGS2 and TNF- α ) ( p ¡ 0.05). TNF- α stimulated expressions of five mediators ( IL6, IL10, IFN γ, PTGS2, and CCL2 ), and AQ and 4-AAQB inhibited IL6 elevation ( p ¡ 0.05). Both components inhibited aromatase expression with/without TNF- α stimulation, with 4-AAQB to be more effective ( p ¡ 0.05). For immune checkpoint CD47, both components inhibited CD47 expression ( p ¡ 0.05), but it did not respond to TNF- α stimulation. For Wnt/ β - catenin signaling downstream genes ( CCND1, C-MYC and AXIN2 ), both components have significant or marginal inhibitory effect on C-MYC in the condition with/without TNF- α stimulation. The luciferase assay demonstrated that both components exhibited inhibitory effect on NF- κ B signaling and Wnt/ β -catenin signaling in the condition without TNF- α stimulation. In conclusion, our results displayed an overall pattern that AQ and 4-AAQB possess potential anti-inflammatory and antitumorigenic functions in MCF-7 breast cancer cells and warranted further in vivo pre-clinical and clinical studies to explore their anticancer properties.