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Enoki, Winter Mushroom · 2010 · Research Support, Non U.S. Gov'T

Medium relevance

Development of a Gene Transfer System for the Mycelia of Flammulina velutipes Fv-1 Strain

Flammulina velutipes

Immune support
SpeciesEnoki, Winter Mushroom
JournalBioscience, biotechnology, and biochemistry
Year2010

Key points

  • To develop a gene transformation method for Flammulina velutipes, we constructed a vector with hph gene under control of the trp1 gene promoter
  • The vector was integrated into protoplast derived from mycelia by the calcium-polyethylene glycol method, as it has not been reported for F. velutipes
  • Transformation efficiency was much improved when transformation was performed by the restriction enzyme mediated integration method

Metadata-grounded summary

Citation abstract

To develop a gene transformation method for Flammulina velutipes, we constructed a vector with hph gene under control of the trp1 gene promoter. The vector was integrated into protoplast derived from mycelia by the calcium-polyethylene glycol method, as it has not been reported for F. velutipes. Transformation efficiency was much improved when transformation was performed by the restriction enzyme mediated integration method.

Citation

Maehara T, Yoshida M, Ito Y, Tomita S, Takabatake K, Ichinose H, et al. (2010). Development of a Gene Transfer System for the Mycelia of Flammulina velutipes Fv-1 Strain. Bioscience, biotechnology, and biochemistry https://doi.org/10.1271/bbb.100021 PMID: 20460695

Open citation