Enoki, Winter Mushroom · 2010 · Research Support, Non U.S. Gov'T
Medium relevanceImprovement of the Transformation Efficiency of Flammulina velutipes Fv-1 Using the Glyceraldehyde-3-phosphate Dehydrogenase Gene Promoter
Flammulina velutipes
Key points
- To improve the expression level of heterologous genes in Flammulina velutipes Fv-1, we constructed new vectors having glyceraldehydes-3-phosphate dehydrogenase (gpd) gene promoter to control the expression of target genes
- When the hygromycin B phosphotransferase (hph) gene from Escherichia coli was controlled by the gpd promoter, transformation efficiency was 3-fold higher than the case of that controlled by the tryptophan synthetase gene (trp1) promoter
From the paper
Abstract
To improve the expression level of heterologous genes in Flammulina velutipes Fv-1, we constructed new vectors having glyceraldehydes-3-phosphate dehydrogenase (gpd) gene promoter to control the expression of target genes. When the hygromycin B phosphotransferase (hph) gene from Escherichia coli was controlled by the gpd promoter, transformation efficiency was 3-fold higher than the case of that controlled by the tryptophan synthetase gene (trp1) promoter.
Citation
Maehara T, Tomita S, Takabatake K, Kaneko S (2010). Improvement of the Transformation Efficiency of Flammulina velutipes Fv-1 Using the Glyceraldehyde-3-phosphate Dehydrogenase Gene Promoter. Bioscience, biotechnology, and biochemistry https://doi.org/10.1271/bbb.100556 PMID: 21150114
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