The White-Rot Fungus Pleurotus ostreatus Transformant Overproduced Intracellular cAMP and Laccase

Pleurotus ostreatus

SpeciesOyster Mushroom

JournalBioscience, Biotechnology, and Biochemistry

Year2013

Key points

Transformation of Pleurotus ostreatus PC9 with the mutated heterotrimeric G protein alpha subunit (Gα) gene resulted in higher laccase (Lac) activity and intracellular cyclic adenosine monophosphate (cAMP) concentrations as compared to those in wild-type PC9
The transformant also exhibited higher Lac activity than the wild type when cultured in a medium containing known Lac inducers CuSO4 and ferulic acid

From the paper

Abstract

Transformation of Pleurotus ostreatus PC9 with the mutated heterotrimeric G protein alpha subunit (Gα) gene resulted in higher laccase (Lac) activity and intracellular cyclic adenosine monophosphate (cAMP) concentrations as compared to those in wild-type PC9. The transformant also exhibited higher Lac activity than the wild type when cultured in a medium containing known Lac inducers CuSO4 and ferulic acid.

Citation

Yuki YAO Takaiku SAKAMOTO Yoichi HONDA Yasuyuki KAGOTANI Kousuke IZUMITSU Kazumi SUZUKI Toshikazu IRIE (2013). The White-Rot Fungus Pleurotus ostreatus Transformant Overproduced Intracellular cAMP and Laccase. Bioscience, Biotechnology, and Biochemistry https://doi.org/10.1271/bbb.130470

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