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Nameko, Nametake · 2002 · Journal Article

Medium relevance

Gene Expression during Pi Deficiency in Pholiota nameko: Accumulation of mRNAs for Two Transporters

Pholiota microspora

Immune support
SpeciesNameko, Nametake
JournalBioscience, biotechnology, and biochemistry
Year2002

Key points

  • The effects of Pi deficiency on gene expression in Pholiota nameko were examined
  • A cDNA library was constructed from poly(A)+ RNA isolated from mycelia cultured in Pi-depleted (P-) media, and 150 clones corresponding to Pi deficiency-inducible (pdi) genes were selected by differential hybridization with probes prepared from poly(A)+ RNAs from the mycelia cultured in the Pi-supplied (P+) and P- media
  • These clones were considered to derive from 31 genes by cross-hybridization
  • Northern blot analysis showed that these pdi genes were expressed in various patterns during Pi deficiency
  • Among the clones, the DNA sequences of pdi85 and pdi343 were analyzed
  • The deduced amino acid sequences indicated that they have structural similarities to Pi and metabolite transporters

Metadata-grounded summary

Citation abstract

The effects of Pi deficiency on gene expression in Pholiota nameko were examined. A cDNA library was constructed from poly(A)+ RNA isolated from mycelia cultured in Pi-depleted (P-) media, and 150 clones corresponding to Pi deficiency-inducible (pdi) genes were selected by differential hybridization with probes prepared from poly(A)+ RNAs from the mycelia cultured in the Pi-supplied (P+) and P- media. These clones were considered to derive from 31 genes by cross-hybridization. Northern blot analysis showed that these pdi genes were expressed in various patterns during Pi deficiency. Among the clones, the DNA sequences of pdi85 and pdi343 were analyzed. The deduced amino acid sequences indicated that they have structural similarities to Pi and metabolite transporters.

Citation

Tasaki Y, Kamiya Y, Azwan A, Hara T, Joh T (2002). Gene Expression during Pi Deficiency in Pholiota nameko: Accumulation of mRNAs for Two Transporters. Bioscience, biotechnology, and biochemistry https://doi.org/10.1271/bbb.66.790 PMID: 12036052

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