Back to search

Shiitake, Hua Gu · 2003 · Journal Article

Medium relevance

Construction of a Homologous Selectable Marker Gene for Lentinula edodes Transformation

Lentinula edodes

SpeciesShiitake, Hua Gu
JournalBioscience, Biotechnology, and Biochemistry
Year2003

Key points

  • We cloned a gene for the iron sulfur protein (Ip) subunit from an edible mushroom, Lentinula edodes, and introduced a point mutation that confers carboxin resistance into it
  • The mutant gene successfully transformed L. edodes with high efficiency (9 transformants/2.5 microg vector DNA)
  • Restriction enzyme-mediated integration (REMI) increased the transformation efficiency by about two-fold

From the paper

Abstract

We cloned a gene for the iron sulfur protein (Ip) subunit from an edible mushroom, Lentinula edodes, and introduced a point mutation that confers carboxin resistance into it. The mutant gene successfully transformed L. edodes with high efficiency (9 transformants/2.5 microg vector DNA). Restriction enzyme-mediated integration (REMI) increased the transformation efficiency by about two-fold.

Citation

Toshikazu IRIE Toshitsugu SATO Kumiko SAITO Yoichi HONDA Takashi WATANABE Masaaki KUWAHARA Hitoshi ENEI (2003). Construction of a Homologous Selectable Marker Gene for Lentinula edodes Transformation. Bioscience, Biotechnology, and Biochemistry https://doi.org/10.1271/bbb.67.2006

Open citation