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Shiitake, Hua Gu · 2004 · Journal Article

Medium relevance

σmarY1, the LTR of the gypsy-Type Retroelement marY1 from the Basidiomycete Tricholoma matsutake, Allows Multicopy DNA Integration in Lentinula edodes

Lentinula edodes

SpeciesShiitake, Hua Gu
JournalBioscience, Biotechnology, and Biochemistry
Year2004

Key points

  • sigma marY1 is the LTR of the retroelement marY1 from the homobasidiomycete Tricholoma matsutake
  • Upon integration through transformation, pLC1-hph carrying a sigma marY1 derivative, sigma* marY1, conferred the hygromycin-resistant phenotype stronger than the vector without sigma* marY1 on Lentinula edodes
  • Based on the densitometric analysis after Southern hybridization, a copy number of the former construct integrated in the genome is much higher than that of the latter
  • We conclude that sigma marY1 allows multicopy DNA integration and will be useful in the genetic research on this fungal group

From the paper

Abstract

sigma marY1 is the LTR of the retroelement marY1 from the homobasidiomycete Tricholoma matsutake. Upon integration through transformation, pLC1-hph carrying a sigma marY1 derivative, sigma* marY1, conferred the hygromycin-resistant phenotype stronger than the vector without sigma* marY1 on Lentinula edodes. Based on the densitometric analysis after Southern hybridization, a copy number of the former construct integrated in the genome is much higher than that of the latter. We conclude that sigma marY1 allows multicopy DNA integration and will be useful in the genetic research on this fungal group.

Citation

Hitoshi MURATA Yasumasa MIYAZAKI (2004). σmarY1, the LTR of the gypsy-Type Retroelement marY1 from the Basidiomycete Tricholoma matsutake, Allows Multicopy DNA Integration in Lentinula edodes. Bioscience, Biotechnology, and Biochemistry https://doi.org/10.1271/bbb.68.218

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