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Shiitake, Hua Gu · 2004 · Journal Article

Medium relevance

Catalytic Reaction of Basidiomycete Lentinula edodes Cytochrome P450, Le.CYP1 Enzyme Produced in Yeast

Lentinula edodes

SpeciesShiitake, Hua Gu
JournalBioscience, Biotechnology, and Biochemistry
Year2004

Key points

  • The basidiomycete Lentinula edodes (Le.) cytochrome P450, Le.CYP1 was functionally expressed in Saccharomyces cerevisiae
  • The microsomal fraction containing Le.CYP1 was prepared from the recombinant yeast and the Le.CYP1 was analyzed
  • The 7-ethoxycoumarin and benzo(a)pyrene were found to be the substrates of Le.CYP1 enzyme
  • Le.CYP1 converted 7-ethoxycoumarin to 7-hydroxycoumarin

From the paper

Abstract

The basidiomycete Lentinula edodes (Le.) cytochrome P450, Le.CYP1 was functionally expressed in Saccharomyces cerevisiae. The microsomal fraction containing Le.CYP1 was prepared from the recombinant yeast and the Le.CYP1 was analyzed. The 7-ethoxycoumarin and benzo(a)pyrene were found to be the substrates of Le.CYP1 enzyme. Le.CYP1 converted 7-ethoxycoumarin to 7-hydroxycoumarin.

Citation

Ryoko AKIYAMA Susumu KAJIWARA Kazuo SHISHIDO (2004). Catalytic Reaction of Basidiomycete Lentinula edodes Cytochrome P450, Le.CYP1 Enzyme Produced in Yeast. Bioscience, Biotechnology, and Biochemistry https://doi.org/10.1271/bbb.68.79

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