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Nameko, Nametake · 1998 · Journal Article

Medium relevance

Isolation and Properties of Glucose-1-phosphatase from Mycelia of Pholiota nameko

Pholiota microspora

Metabolic health
SpeciesNameko, Nametake
JournalBioscience, biotechnology, and biochemistry
Year1998

Key points

  • An acid phosphatase with a very high substrate specificity for glucose-1-phosphate was isolated for the first time from mycelia of Pholiota nameko
  • The molecular weight of the enzyme was estimated to be 31,000 on gel filtration and 35,000 on SDS-PAGE. The activity was inhibited by Cu2+, Hg2+, molybdate, and tartaric acid
  • The sequence of N-terminal 20 amino acid residues was analyzed

Metadata-grounded summary

Citation abstract

An acid phosphatase with a very high substrate specificity for glucose-1-phosphate was isolated for the first time from mycelia of Pholiota nameko. The molecular weight of the enzyme was estimated to be 31,000 on gel filtration and 35,000 on SDS-PAGE. The activity was inhibited by Cu2+, Hg2+, molybdate, and tartaric acid. The sequence of N-terminal 20 amino acid residues was analyzed.

Citation

Joh T, Yazaki J, Suzuki K, Hayakawa T (1998). Isolation and Properties of Glucose-1-phosphatase from Mycelia of Pholiota nameko. Bioscience, biotechnology, and biochemistry https://doi.org/10.1271/bbb.62.2251 PMID: 9972248

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