Back to search

Maitake, Hen of the Woods · 2009 · Research Support, Non U.S. Gov'T

Low relevance

Involvement of Branched Units at Position 6 in the Reactivity of a Unique Variety of β-D-Glucan from Aureobasidium pullulans to Antibodies in Human Sera

Grifola frondosa

Immune support
SpeciesMaitake, Hen of the Woods
JournalBioscience, biotechnology, and biochemistry
Year2009

Key points

  • We recently determined the structure of a unique type of 1,3-beta-D-glucan obtained from Aureobasidium pullulans (AP-FBG) and found that it reacted with the antibodies in human sera
  • The reactivity of AP-FBG to the antibodies was stronger than that of 1,3-beta-D-glucan obtained Grifola frondosa (GRN) but weaker than that of 1,3-beta-D-glucan from Candida albicans (CSBG)
  • Here, we demonstrated that AP-FBG reacted to IgG antibodies, especially those of the subclasses IgG2, IgG1, and IgG3, in human sera
  • Moreover, the results of competitive enzyme-linked immunosorbent assays (ELISAs) using various glucan competitors showed that these IgGs recognized branched chains at position 6
  • This is the first study to report that the branched chains at position 6 of beta-D-glucan strongly contribute to its recognition by antibodies in human sera
  • This high reactivity of AP-FBG to human IgG could be advantageous for the use of this glucan in medicine, e.g., as an immunostimulatory agent

Metadata-grounded summary

Citation abstract

We recently determined the structure of a unique type of 1,3-beta-D-glucan obtained from Aureobasidium pullulans (AP-FBG) and found that it reacted with the antibodies in human sera. The reactivity of AP-FBG to the antibodies was stronger than that of 1,3-beta-D-glucan obtained Grifola frondosa (GRN) but weaker than that of 1,3-beta-D-glucan from Candida albicans (CSBG). Here, we demonstrated that AP-FBG reacted to IgG antibodies, especially those of the subclasses IgG2, IgG1, and IgG3, in human sera. Moreover, the results of competitive enzyme-linked immunosorbent assays (ELISAs) using various glucan competitors showed that these IgGs recognized branched chains at position 6. This is the first study to report that the branched chains at position 6 of beta-D-glucan strongly contribute to its recognition by antibodies in human sera. This high reactivity of AP-FBG to human IgG could be advantageous for the use of this glucan in medicine, e.g., as an immunostimulatory agent.

Citation

Tada R, Tanioka A, Ishibashi K, Adachi Y, Tsubaki K, Ohno N (2009). Involvement of Branched Units at Position 6 in the Reactivity of a Unique Variety of β-D-Glucan from Aureobasidium pullulans to Antibodies in Human Sera. Bioscience, biotechnology, and biochemistry https://doi.org/10.1271/bbb.80860 PMID: 19352033

Open citation