An in vitro evaluation of the cytotoxic potential of medicinal mushrooms against human breast cancer cell lines.

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Citation abstract

Medicinal mushroom extracts, i.e. their dried biomass, have long been known as sources of bioactive compounds with positive effects on the human health. The antioxidant, antigenotoxic, antiviral, and immunomodulatory properties of the commercially available extracts Agaricus blazei auct. non Murrill (AB), Cordyceps sinensis (Berk.) Sacc. (CS), and Immune Assist (IA) have already been documented. This study, studied the influence of these three mushrooms on the viability of cell lines MCF-7, MDA-MB-231, and HS-5. The cytotoxicity of AB, CS, and IA at different concentrations (25, 50, 100, 200, 400 and 800 μg/mL) was evaluated using the MTT assay. The results showed that AB was the most effective and induced cytotoxicity in both cancer cell lines, with IC 50 values of 96.7 μg/mL for MCF-7 and 368.4 μg/mL for MDA-MB-231. After treatment with CS and IA, the half-maximal inhibitory concentration was reached only in MDA- MB-231 cells (IC 50 =613 μg/mL for CS and 343.3 μg/mL for IA). We have shown here that AB, CS and IA can suppress the growth of MCF-7 and MDA-MB-231 cell lines, while affecting the survival of healthy HS-5 cells to a much lesser extent. Our in vitro results suggested that AB, CS and IA are promising natural sources with potential anticancer activity.

Ekstrakti gljiva za medicinsku upotrebu, odnosno njihova osušena biomasa, odavno su poznati kao izvori bioaktivnih spojeva s pozitivnim učinkom na ljudsko zdravlje. Antioksidacijska, antigenotoksična, antivirusna i imunomodulirajuća svojstva komercijalno dostupnih ekstrakata Agaricus blazei auct. non Murrill (AB), Cordyceps sinensis (Berk.) Sacc. (CS) i Immune Assist (IA) već su dugo poznata. Ovim istraživanjem ispitan je učinak tih triju gljiva na održivost staničnih linija MCF-7, MDA-MB-231 i HS-5. Citotoksičnost AB, CS i IA u različitim koncentracijama (25, 50, 100, 200, 400 i 800 μg/mL) procijenjena je MTT testom. Rezultati su pokazali da je AB izazvao najučinkovitiju citotoksičnost u objema staničnim linijama raka, s IC 50 vrijednostima od 96,7 μg/mL za MCF-7 i 368,4 μg/mL za MDA-MB-231. Nakon tretmana s CS i IA polumaksimalna inhibitorna koncentracija postignuta je samo u stanicama MDA-MB-231 (IC 50 =613 μg/mL za CS i 343,3 μg/mL za IA). Ovo je istraživanje pokazalo da AB, CS i IA mogu donekle suzbiti rast staničnih linija raka bez utjecaja na preživljavanje normalnih HS-5 stanica. Naši rezultati sugeriraju da su AB, CS i IA obećavajući prirodni izvori s potencijalnim djelovanjem protiv raka.