Genome-wide identification of small GTPase gene family members in Lentinula edodes and their expression profiling under adversity stress

From the paper

Abstract

Background: Small GTPase superfamily is a small molecular switch involved inmultiple biological processes of eukaryotes. The main functions of GTPases in fungi include morphogenesis, secondary metabolism, vesicle trafficking, stressresponse, and virulence. Results: A total of 34 the Lentinula edodes small GTPase (Le-small GTPase) family genes were indentified, and they were clustered into 5 subfamilies, namely Rho, Ras, Arf, Rab, and Ran subfamily. Le-small GTPase genes were evenly distributed on 10 chromosomes. The number of amino acids of 34 Lentinula edodes small GTPase proteins ranged from 155 to 627, and all these 34 proteinsexhibited high hydropility and stability. Our quantitative PCR results indicatethat all the Le-small GTPase genes with stress - responsive cis-acting elementsdo respond to various stresses. Conclusions: In this study, the small GTPase genes of Lentinula edodes were more similar to those of 2 basidiomycetes than to those of 5 ascomycetes. The small GTPase protein genes of Lentinula edodes were evenly distributed on tenchromosomes without tandem repeat events. The multiple stress-responsivecis-acting elements were found to be on the promoter of the small GTPase genesof Lentinula edodes. Our findings may deepen the understanding of smallGTPase proteins in Lentinula edodes and provide valuable reference for furtherresearch on stress response mechanism of small GTPase protein family in morefungi