On site discrimination between two closely related commercial strains of Oyster mushroom using a loop-mediated isothermal amplification (LAMP) test

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Abstract

Abstract This article describes the development of tools for the on-site identification of two closely related sporeless strains of Oyster mushroom, using either the LAMP technique or a modification of that technique. It allows for fast (within 30 minutes) identification of the commercially used strains SPOPPO and ALLERPO. Fast on-site answers on strain identity can be important when experiencing unexpected strain behavior or when strains are of suspect origin. Both strains are discriminated from sporulating strains by a LAMP reaction on the intact version of the msh4 gene; sporeless strains contain a msh4 gene with a large insert that renders the associated protein inactive. SPOPPO and ALLERPO are distinguished from each other by LAMP reactions that target genomic regions with strain specific recombinations.