Back to search

Shiitake, Hua Gu · 2017 · Journal Article

Low relevance

Rapid Identification Method of Omphalotus japonicus by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP)

Lentinula edodes

Immune supportGut & microbiome
SpeciesShiitake, Hua Gu
JournalShokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan
Year2017

Key points

  • Omphalotus japonicus is a poisonous mushroom that grows in Japan
  • It can be mistaken for edible mushrooms (Shiitake, Hiratake and Mukitake), and if ingested, it causes food poisoning within 30 min to 1 hr
  • We established a rapid detection method using PCR-RFLP to identify O. japonicus by restriction digestion of the amplified ITS region
  • By using Sau96I, Bpu10I, SfcI or DrdI/HincII as a restriction enzyme, it was possible to rapidly identify and discriminate O. japonicus based on the fragment length
  • This study also provided a short PCR-RFLP system comprising amplification and digestion of a short 200-bp DNA fragment within the ITS region
  • The system could identify and discriminate O. japonicus after in vitro gastric digestion of native and heated mushroom samples as a model of food poisoning

Metadata-grounded summary

Citation abstract

Omphalotus japonicus is a poisonous mushroom that grows in Japan. It can be mistaken for edible mushrooms (Shiitake, Hiratake and Mukitake), and if ingested, it causes food poisoning within 30 min to 1 hr. We established a rapid detection method using PCR-RFLP to identify O. japonicus by restriction digestion of the amplified ITS region. By using Sau96I, Bpu10I, SfcI or DrdI/HincII as a restriction enzyme, it was possible to rapidly identify and discriminate O. japonicus based on the fragment length. This study also provided a short PCR-RFLP system comprising amplification and digestion of a short 200-bp DNA fragment within the ITS region. The system could identify and discriminate O. japonicus after in vitro gastric digestion of native and heated mushroom samples as a model of food poisoning. In addition, a confirmatory assay using real-time PCR was developed to achieve more sensitive detection of O. japonicus.

Citation

Sugano Y, Sakata K, Nakamura K, Noguchi A, Fukuda N, Suzuki T, et al. (2017). Rapid Identification Method of Omphalotus japonicus by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP). Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan https://doi.org/10.3358/shokueishi.58.113 PMID: 28690301

Open citation