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Cordyceps, Caterpillar Fungus · 2022 · Journal Article

Medium relevance

Extraction, Purification, Physicochemical Properties, and Activity of a New Polysaccharide From Cordyceps cicadae.

Ophiocordyceps sinensis

Immune supportEnergy & fatigueRespiratory
SpeciesCordyceps, Caterpillar Fungus
JournalFrontiers in nutrition
Year2022

Key points

  • The polysaccharides from C. cicadae were extracted by ultrasonically-assisted enzymatic extraction (UAEE)
  • Response surface analysis was used to determine the optimum parameters as follows: addition of enzymes, 0.71%; extraction temperature, 60°C; extraction time, 18 min; liquid-solid ratio, 46:1 (mL/g)
  • The extraction yield of polysaccharide was 3.66 ± 0.87%
  • The analysis results showed that the molar ratio of galactose, glucose, and mannose in JCH-a1 cells (60.7 kDa) was 0.89:1:0.39
  • Moreover, JCH-a1 showed strong antioxidant activity and acted as a strong inhibitor of α-glucosidase in vitro
  • The present study might provide a basis for further study of JCH-a1 as an antioxidant and hypoglycemic food or drug

Metadata-grounded summary

Citation abstract

The polysaccharides from C. cicadae were extracted by ultrasonically-assisted enzymatic extraction (UAEE). Response surface analysis was used to determine the optimum parameters as follows: addition of enzymes, 0.71%; extraction temperature, 60°C; extraction time, 18 min; liquid-solid ratio, 46:1 (mL/g). The extraction yield of polysaccharide was 3.66 ± 0.87%. A novel polysaccharide fraction (JCH-a1) from C. cicadae was extracted and then purified by cellulose DEAE-32 and Sephadex G-100 anion exchange chromatography. The analysis results showed that the molar ratio of galactose, glucose, and mannose in JCH-a1 cells (60.7 kDa) was 0.89:1:0.39. JCH-a1 with a triple helix contains more α-glycosides and has strong thermal stability. Moreover, JCH-a1 showed strong antioxidant activity and acted as a strong inhibitor of α-glucosidase in vitro. In addition, JCH-a1 can prolong the lifespan of C. elegans. The present study might provide a basis for further study of JCH-a1 as an antioxidant and hypoglycemic food or drug.

Citation

Tang Z, Lin W, Chen Y, Feng S, Qin Y, Xiao Y, et al. (2022). Extraction, Purification, Physicochemical Properties, and Activity of a New Polysaccharide From Cordyceps cicadae. Frontiers in nutrition https://doi.org/10.3389/fnut.2022.911310 PMID: 35757258

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