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Snow Fungus, Silver Ear · 2016 · Research Article

Medium relevance

A Method for Determining the Content of Glycoproteins in Biological Samples.

Tremella fuciformis

Gut & microbiomeSkin & hydration
SpeciesSnow Fungus, Silver Ear
JournalMolecules (Basel, Switzerland)
Year2016

Key points

  • The glycoprotein purified from the mycelium extract of Tremella fuciformis was marked with iodine through the iodine substitution reaction
  • The content of iodine, which is indicative of the amount of the marked tremella glycoprotein (ITG), was detected with Inductively coupled plasma mass spectrometry (ICP-MS)
  • The method was found to be stable, sensitive, and accurate at detecting the content of iodine-substituted glycoprotein, and was used in the quantitative analysis of biological samples, including blood and organs
  • Different biological samples were collected from rats after oral administration of ITG, and were tested for iodine content by ICP-MS to calculate the amount of ITG in the samples
  • The results suggested that ICP-MS is a sensitive, stable, and accurate method for detection of iodinated glycoproteins in blood and organs

Metadata-grounded summary

Citation abstract

The glycoprotein purified from the mycelium extract of Tremella fuciformis was marked with iodine through the iodine substitution reaction. The content of iodine, which is indicative of the amount of the marked tremella glycoprotein (ITG), was detected with Inductively coupled plasma mass spectrometry (ICP-MS). The method was found to be stable, sensitive, and accurate at detecting the content of iodine-substituted glycoprotein, and was used in the quantitative analysis of biological samples, including blood and organs. Different biological samples were collected from rats after oral administration of ITG, and were tested for iodine content by ICP-MS to calculate the amount of ITG in the samples. The results suggested that ICP-MS is a sensitive, stable, and accurate method for detection of iodinated glycoproteins in blood and organs.

Citation

Gao Y, Xu D, Li H, Yang X, Wang M, Gao Q (2016). A Method for Determining the Content of Glycoproteins in Biological Samples. Molecules (Basel, Switzerland) https://doi.org/10.3390/molecules21121625 PMID: 27898038

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