Antimicrobial, Antioxidant and Antiproliferative Secondary Metabolites from Inonotus nidus-pici.

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Citation abstract

Inonotus nidus-pici is a sterile conk which produces macrofungus, a neglected Central-Eastern European relative of the prized Inonotus obliquus, also known as chaga. Investigation of the methanol extract of the poroid fungus I. nidus-pici resulted in the isolation of citropremide ( 1 ), 3,4-dihydroxybenzalacetone ( 2 ), lanosterol ( 3 ), ergost-6,8,22-trien-3β-ol ( 4 ), and ergosterol peroxide ( 5 ). The structures of fungal compounds were determined on the basis of one- and two-dimensional NMR and MS spectroscopic analysis. Compounds 1 - 2 and 4 - 5 were evaluated for their antioxidant and antimicrobial properties against several bacterial and fungal strains. 3,4-dihydroxybenzalacetone ( 2 ) and ergost-6,8,22-trien-3β-ol ( 4 ) demonstrated moderate antimicrobial activity, while the former possessed notable antioxidant activity in DPPH assay. The antiproliferative examinations performed on three human cancer (MES-SA, MES-SA/Dx5, A431) cell lines demonstrated that compounds 4 and 5 have notable cytotoxic activity with IC values in micromolar range. The current study represents the first report on the chemical profile of I. nidus-pici, providing a comprehensive study on the isolation and structure determination of bioactive secondary metabolites of this macrofungus.