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Niu-Chang-Chih · 2022 · Research Article

High relevance

Anti-Inflammatory Constituents of Antrodia camphorata on RAW 264.7 Cells Induced by Polyinosinic-Polycytidylic Acid.

Antrodia camphorata

OncologyCognition & nervesLiver support
SpeciesNiu-Chang-Chih
JournalMolecules (Basel, Switzerland)
Year2022

Key points

  • Antrodia camphorata is an endemic mushroom in Taiwan
  • This study was designed to screen anti-inflammatory compounds from the methanolic extract of the mycelium of A. camphorata on nitric oxide (NO) production in RAW 264.7 cells induced by polyinosinic-polycytidylic acid (poly I:C), a synthetic analog of double-stranded RNA (dsRNA) known to be present in viral infection
  • A combination of bioactivity-guided isolation with an NMR-based identification led to the isolation of 4-acetylantroquinonol B ( 1 ), along with seven compounds
  • The structure of new compounds ( 4 and 5 ) was elucidated by spectroscopic experiments, including MS, IR, and NMR analysis
  • The anti-inflammatory activity of all isolated compounds was assessed at non-cytotoxic concentrations. 4-Acetylantroquinonol B ( 1 ) was the most potent compound against poly I:C-induced NO production in RAW 264.7 cells with an IC 50 value of 0.57 ± 0.06 μM

Metadata-grounded summary

Citation abstract

Antrodia camphorata is an endemic mushroom in Taiwan. This study was designed to screen anti-inflammatory compounds from the methanolic extract of the mycelium of A. camphorata on nitric oxide (NO) production in RAW 264.7 cells induced by polyinosinic-polycytidylic acid (poly I:C), a synthetic analog of double-stranded RNA (dsRNA) known to be present in viral infection. A combination of bioactivity-guided isolation with an NMR-based identification led to the isolation of 4-acetylantroquinonol B ( 1 ), along with seven compounds. The structure of new compounds ( 4 and 5 ) was elucidated by spectroscopic experiments, including MS, IR, and NMR analysis. The anti-inflammatory activity of all isolated compounds was assessed at non-cytotoxic concentrations. 4-Acetylantroquinonol B ( 1 ) was the most potent compound against poly I:C-induced NO production in RAW 264.7 cells with an IC 50 value of 0.57 ± 0.06 μM.

Citation

Tu PC, Jiang WP, Lin MK, Huang GJ, Li YJ, Kuo YH (2022). Anti-Inflammatory Constituents of Antrodia camphorata on RAW 264.7 Cells Induced by Polyinosinic-Polycytidylic Acid. Molecules (Basel, Switzerland) https://doi.org/10.3390/molecules27165320 PMID: 36014558

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