Enoki, Winter Mushroom · 2006 · Brief Report
High relevanceAgrobacterium-mediated Transformation of the Winter Mushroom, Flammulina velutipes.
Flammulina velutipes
Key points
- Flammulina velutipes was transformed efficiently by Agrobacterium-mediated transformation system
- The transformation frequency was about 16% with the gill tissues of the fungal fruiting body
- Southern hybridization and genetic analysis suggest that the introduced DNA was inserted onto different locations of the fungal genome, and inherited stably to the next generation via basidiospores
- Transformation or gene tagging with Agrobacterium T-DNA based vector should be useful for wide ranges of genetic or molecular biological studies of the mushroom
Metadata-grounded summary
Citation abstract
Flammulina velutipes was transformed efficiently by Agrobacterium-mediated transformation system. The transformation frequency was about 16% with the gill tissues of the fungal fruiting body. Southern hybridization and genetic analysis suggest that the introduced DNA was inserted onto different locations of the fungal genome, and inherited stably to the next generation via basidiospores. Transformation or gene tagging with Agrobacterium T-DNA based vector should be useful for wide ranges of genetic or molecular biological studies of the mushroom.
Citation
Cho JH, Lee SE, Chang WB, Cha JS (2006). Agrobacterium-mediated Transformation of the Winter Mushroom, Flammulina velutipes. Mycobiology https://doi.org/10.4489/MYCO.2006.34.2.104 PMID: 24039479
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