Wood Ear, Kikurage · 2010 · Brief Report
Medium relevanceDevelopment of Detection Methods for Cellulolytic Activity of Auricularia auricula-judae.
Auricularia auricula-judae
Key points
- To obtain basic information on the detection of cellulolytic activity in Auricularia auricula-judae, the influences of dye reagent, pH, and temperature were assessed
- Chromogenic dye (congo red, phenol red, remazol brilliant blue, and trypan blue) was individually incorporated into a medium containing either carboxymethyl-cellulose, Avicel, or D-cellobiose as a polysaccharide carbon substrate
- The other assessments utilized pHs ranging from 4.5 to 8.0 and temperatures from 15~35℃. Overall, when A. auricula-judae species were transferred onto media contained Congo red and adjusted pH 7.0 and then incubated at 25℃ for 5 days, the clear zone indicative of cellulolytic activity was more pronounced
Metadata-grounded summary
Citation abstract
To obtain basic information on the detection of cellulolytic activity in Auricularia auricula-judae, the influences of dye reagent, pH, and temperature were assessed. Chromogenic dye (congo red, phenol red, remazol brilliant blue, and trypan blue) was individually incorporated into a medium containing either carboxymethyl-cellulose, Avicel, or D-cellobiose as a polysaccharide carbon substrate. The other assessments utilized pHs ranging from 4.5 to 8.0 and temperatures from 15~35℃. Overall, when A. auricula-judae species were transferred onto media contained Congo red and adjusted pH 7.0 and then incubated at 25℃ for 5 days, the clear zone indicative of cellulolytic activity was more pronounced.
Citation
Jo WS, Bae SH, Choi SY, Park SD, Yoo YB, Park SC (2010). Development of Detection Methods for Cellulolytic Activity of Auricularia auricula-judae. Mycobiology https://doi.org/10.4489/MYCO.2010.38.1.074 PMID: 23956630
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