King Oyster, Eryngii · 2010 · Brief Report
High relevanceScreening of Cell Cycle-Related Genes of Pleurotus eryngii Using Yeast Mutant Strains.
Pleurotus eryngii
Key points
- Temperature-sensitive yeast mutants were used to screen for cell cycle-related genes from Pleurotus eryngii genomic DNA. A mushroom genomic DNA library was established and each gene was screened for the ability to rescue seven Saccharomyces cerevisiae temperature-sensitive strains
- Hundreds of yeast transformants were selected at restrictive temperatures over 30℃. Plasmids from the transformants that survived were isolated and transformed back into their host strains
- The temperature sensitivity of the resulting transformants was tested from 30℃ to 37℃. Ten DNA fragments from P. eryngii were able to rescue yeast temperature-sensitive strains, and their DNA sequences were determined
Metadata-grounded summary
Citation abstract
Temperature-sensitive yeast mutants were used to screen for cell cycle-related genes from Pleurotus eryngii genomic DNA. A mushroom genomic DNA library was established and each gene was screened for the ability to rescue seven Saccharomyces cerevisiae temperature-sensitive strains. Hundreds of yeast transformants were selected at restrictive temperatures over 30℃. Plasmids from the transformants that survived were isolated and transformed back into their host strains. The temperature sensitivity of the resulting transformants was tested from 30℃ to 37℃. Ten DNA fragments from P. eryngii were able to rescue yeast temperature-sensitive strains, and their DNA sequences were determined.
Citation
Shi S, Ro HS (2010). Screening of Cell Cycle-Related Genes of Pleurotus eryngii Using Yeast Mutant Strains. Mycobiology https://doi.org/10.4489/MYCO.2010.38.1.070 PMID: 23956629
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