Isolation of 1',3'-dilinolenoyl'-2'-linoleoylglycerol with tyrosinase inhibitory activity from Flammulina velutipes.

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Citation abstract

This study was carried out to evaluate the inhibitory effect of Flammulina velutipes extracts on tyrosinase activity and to identify its biologically active component. The ethyl acetate and n-butanol extracts showed potent tyrosinase inhibitory activities. Subsequently, fractions of the nbutanol extract showed only a partial tyrosinase inhibitory activity. The most active compound of tyrosinase inhibitory activity was identified from the ethyl acetate extract as 1',3'-dilinolenoyl-2'-linoleoylglycerol (LnLLn) by comparing its mass, 1H-, and 13C-NMR spectral data with those previously reported in the literature. LnLLn showed tyrosinase inhibitory activity with an IC50 value of 16.1 mug/ml. These results suggest that the ethyl acetate extract of F. velutipes could be applicable for the development of a new whitening agent.