Snow Fungus, Silver Ear · 2017 · Brief Report
High relevanceAn Improved Total RNA Extraction Method for White Jelly Mushroom Tremella fuciformis Rich in Polysaccharides.
Tremella fuciformis
Key points
- An improved method for extracting high quality and quantity RNA from a jelly mushroom and a dimorphic fungus- Tremella fuciformis which is especially rich in polysaccharides, is described
- RNA was extracted from T. fuciformis mycelium M1332 and its parental monokaryotic yeast-like cells Y13 and Y32
- The A260/280 and A260/230 ratios were both approximately 2, and the RNA integrity number was larger than 8.9
- The yields of RNA were between 108 and 213 µg/g fresh wt
- Downstream molecular applications including reverse transcriptional PCR and quantitative real-time PCR were also performed
- This protocol is reliable and may be widely applicable for total RNA extraction from other jelly mushrooms or filamentous fungi rich in polysaccharides
Metadata-grounded summary
Citation abstract
An improved method for extracting high quality and quantity RNA from a jelly mushroom and a dimorphic fungus- Tremella fuciformis which is especially rich in polysaccharides, is described. RNA was extracted from T. fuciformis mycelium M1332 and its parental monokaryotic yeast-like cells Y13 and Y32. The A260/280 and A260/230 ratios were both approximately 2, and the RNA integrity number was larger than 8.9. The yields of RNA were between 108 and 213 µg/g fresh wt. Downstream molecular applications including reverse transcriptional PCR and quantitative real-time PCR were also performed. This protocol is reliable and may be widely applicable for total RNA extraction from other jelly mushrooms or filamentous fungi rich in polysaccharides.
Citation
Zhu H, Sun X, Liu D, Zheng L, Chen L, Ma A (2017). An Improved Total RNA Extraction Method for White Jelly Mushroom Tremella fuciformis Rich in Polysaccharides. Mycobiology https://doi.org/10.5941/MYCO.2017.45.4.434 PMID: 29371814
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