King Oyster, Eryngii · 2012 · Research Article
Low relevanceCloning and sequence analysis of the cellobiohydrolase I genes from some basidiomycetes.
Pleurotus eryngii
Key points
- Genes encoding the cellobiohydrolase enzyme (CBHI), designated as cbhI, were isolated from the basidiomycetes Auricularia fuscosuccinea, Pleurotus giganteus, P. eryngii, P. ostreatus, and P. sajor-caju
- Initially, the fungal genomic DNA was extracted using a modified cetyltrimethyl ammonium bromide (CTAB) protocol and used as a DNA template
- The cbhI genes were then amplified and cloned using the pGEM-T Easy Vector Systems
- The sizes of these PCR amplicons were between 700~800 bp
- The DNA sequences obtained were similar showing high identity to the cbhI gene family
- The deduced amino acid sequences exhibited significant similarity to those of fungal CBHI enzymes belonging to glycosyl hydrolase family 7
Metadata-grounded summary
Citation abstract
Genes encoding the cellobiohydrolase enzyme (CBHI), designated as cbhI, were isolated from the basidiomycetes Auricularia fuscosuccinea, Pleurotus giganteus, P. eryngii, P. ostreatus, and P. sajor-caju. Initially, the fungal genomic DNA was extracted using a modified cetyltrimethyl ammonium bromide (CTAB) protocol and used as a DNA template. The cbhI genes were then amplified and cloned using the pGEM-T Easy Vector Systems. The sizes of these PCR amplicons were between 700~800 bp. The DNA sequences obtained were similar showing high identity to the cbhI gene family. These cbhI genes were partial consisting of three coding regions and two introns. The deduced amino acid sequences exhibited significant similarity to those of fungal CBHI enzymes belonging to glycosyl hydrolase family 7.
Citation
Chukeatirote E, Maharachchikumbura SS, Wongkham S, Sysouphanthong P, Phookamsak R, Hyde KD (2012). Cloning and sequence analysis of the cellobiohydrolase I genes from some basidiomycetes. Mycobiology https://doi.org/10.5941/MYCO.2012.40.2.107 PMID: 22870052
Open citation