Artificial nutrient medium for Phellinus linteus fluid culture and method for fermentation of Phellinus linteus polysaccharide

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Abstract

The invention provides a synthetic medium for culturing a phellinus igniarius liquid. The synthetic medium takes glucose as a carbon source and 20 kinds of amino acid as a nitrogen source, and consists of inorganic salt and vitamin. The ratio of carbon to nitrogen is designed as 50 to 1 and the specific concentration g/l is as follows: the glucose: 30 to 80 grams glutamic acid: 0.1 to 1.5 grams glutamine: 0.1 to 1.5 grams aspartic acid: 0.1 to 1.5 grams asparagines: 0.1 to 1.5 grams glutamic acid: 0.01 to 0.1 gram tryptophan: 0.01 to 0.1 gram valine: 0.01 to 0.1 gram alanine: 0.01 to 0.1 gram etc. During the whole culture process of the synthetic medium, phellinus igniarius mycelium is quick in growth the yield of the phellinus igniarius mycelium can reach 20 grams per liter to the highest degree simultaneously no agricultural and sideline product is introduced as the carbon source and the nitrogen source substances such as proteins in the medium, medium compositions and so on are not carried phellinus igniarius exopolysaccharide produced through fermentation has high purity and stable quality and the yield of phellinus igniarius polysaccharide can reach 0.198 gram per liter to the highest degree. The synthetic medium has the advantages of clear compositions, stable quality and so on, and can be used for mass culture of the phellinus igniarius liquid and research of the regulation and control rule of polysaccharide anabolism.