Method for technical evaluation of hedgehog fungus Houjie bacterial with numerator mark

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Abstract

The invention relates to a method for identifying the houjie strain of hericium erinaceus by making use of a molecular marker technology, which comprises the culture and collection of mycelium, the extraction of genomic DNA, the detection establishment of SCAR-PCR molecular markers and the detection of SCAR-PCR products. The method provided by the invention for identifying the houjie strain of the hericium erinaceus by making use of the molecular marker technology is high in sensitivity and less in the required quantity of DNA. Compared with the conventional morphologic detection, antagonistic test and fruiting test, the method has the advantages of short detection time, high accuracy, easy judgment, good repetition and the like, provides a rapid and effective system of bacteria identification for the utilization of the germplasm resources of the hericium erinaceus and the registration work of new species, and is very important for scientific research and production.