Method for technical evaluation of hedgehog fungus bacterial Houwang with numerator mark

From the paper

Abstract

The invention relates to a method for identifying the strain of a hericium erinaceus king by making use of a molecular marker technology, which comprises the culture and collection of mycelium, the extraction of genomic DNA, the detection establishment of SCAR-PCR molecular markers and the detection of SCAR-PCR products. The invention adopts two pairs of different primer kings: F1/R1 and F2/R2, to detect the SCAR-PCR molecular markers of the strains to be tested and the SCAR-PCR products when the king F1/R1 is used for detecting the primers, the molecular weight of the detection result of the SCAR-PCR products shown in a DNA map is a specific 1012bp DNA band which is the symbol of the strain of the hericium erinaceus king when the king F2/R2 is used for detecting the primers, the molecular weight of the detection result of the SCAR-PCR products shown in a DNA map is a specific 371bp DNA band which is the symbol of the strain of the hericium erinaceus king.