Inonotus obliquus extracellular and incellular mixed exopolysaccharide and preparation method thereof and pharmaceutical use

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Abstract

The invention relates to ionotus obliquus extracellular and intracellular mixing polysaccharide, a method for preparing the same and medical application, and belongs to the field of medicine. Under aseptic conditions, slant strain is inoculated in a fresh petri dish with comprehensive solid culture medium, after the first-level fermentation and the second-level fermentation, the product of the second-level fermentation is subjected to suction filtering to obtain mycelium of ionotus obliquus and fermentation liquid, and after concentration and aqueous extraction-alcohol precipitation, the extracellular and intracellular mixing polysaccharid is obtained. In the invention, the fermentation product obtained by the liquid submerged fermentation also contains medical active components, the growth period is short, and the invention can be used as an effective method for obtaining active components. Based on tests, in the invention, the mixing coarse polysaccharide is extracted from the mycelium of the ionotus obliquus and the fermentation liquid of the mycelium of the ionotus obliquus, and the anti-tumor activity of the mixing polysaccharide is researched, and the ionotus obliquus extracellular and intracellular mixing polysaccharide has the characteristics of high tumor inhibition activity and low toxicity.