Preparation method of inonotus obilquus as alpha-glucosidase inhibitors

From the paper

Abstract

The invention discloses a preparation method of an Inonotus obliquus extract which can be used as an Alpha-glucosaccharase inhibitor. In the preparation method, ethanol is firstly adopted to heat and reflow the Inonotus obliquus so as to obtain an ethanol extract, then the ethanol extract is fully dispersed into distilled water and is extracted by sherwood oil so as to remove low-polarity components in the extract, which have no inhibition activity to the Alpha-glucosaccharase and then the raffinate is extracted by ethyl acetate, thereby obtaining the extract which has strong inhibition activity to the Alpha-glucosaccharase and the yield rate is 2 to 4 percent. Experiment proves that the Inonotus obliquus extract obtained by the preparation method provided by the invention has very strong inhibition activity to the Alpha-glucosaccharase when the dosage is 400 Mug, the inhibition rate can reach 80.5 percent, with the IC50 of 103.9 Mug/ml therefore, the method can be used for developing sugar-reducing medicament. Moreover, the preparation method also has the advantages of simple technique, convenient operation, and the like.