Preparation and use of recombinant gold needle mushroom immunomodulatory protein

From the paper

Abstract

The invention discloses a preparation method and application of a recombinant flammulina velutipes immunomodulatory protein that is expressed by Pasteur pichia pastoris, and the preparation method comprises following steps: (1) an encoding FIP-fve gene is cloned (2) a recombinant Pasteur pichia pastoris expression carrier is constructed (3) a recombinant Pasteur pichia pastoris transformant is obtained and (4) the recombinant FIP-fve gene is induction expressed and purified. The preparation method clones an encoding flammulina velutipes immunomodulatory protein, constructs the Pasteur pichia pastoris expression carrier, and consequently obtains the recombinant flammulina velutipes immunomodulatory protein with high yield and high purity. The yield of the recombinant protein that is obtained by the 5-liter fermentation technique reaches 300mg/L to 350mg/L, the purity reaches over 90 percent, and the purified recombinant flammulina velutipes immunomodulatory protein is detected to have the same biologic features and physiological immune activity with natural flammulina velutipes immunomodulatory protein, has procoagulant activity and the promoting functions of mouse lymphocyte proliferation as well as interleukin and interferon secretion, and can induce tumor cell apoptosis.