Artificial cultivation method for sclerotium and fruiting body of Inonotus obliquus

From the paper

Abstract

The present invention discloses an artificial cultivation method of sclerotium and sporophore of innotus obliquus, wherein the method comprises the following steps: after executing expanding culture to the mother seed of innotus obliquus on the enriched PDA culture medium for obtaining the stock seed inoculating the stock seed in the cultivated species culture medium for cultivating unit the mycelium is overgrown. The culture bag which is overgrown with mycelium is transferred to a mushroom generating room for mushroom generating culture, wherein the mushroom generating culture is executed below the following conditions: relative air moisture of air for 80%, culture temperature for 25 DEG C, and illumination intensity for 1001x. The artificial cultivation method of the invention can remarkably increase the growing speed of mycelium of sclerotium. The average growth rate of mycelium in one day can obtain 0.67mm/d furthest, and the growth of mycelium is tidy and compact. When the bag is filled with mycelium, the budding time of fungus bag is early. The number of generated sclerotium and sporophore is large. The production volume is high and the quality is excellent, wherein, the biological efficiency of sclerotium reaches up to 67%.