Preparation method of pholiota nameko polysaccharide extract

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Abstract

The invention discloses a preparation method of a pholiota nameko polysaccharide extract and provides the preparation method which has the advantages of scale production, simple process and low cost. The preparation method comprises the following steps: soaking a pholiota nameko dry product in hot water, and fully swelling beating, and obtaining pholiota nameko slurry adding trypsinase in the pholiota nameko slurry, and keeping the temperature for 1h at the temperature of 37 DEG C further adding cellulase, and keeping the temperature for 2h at the temperature of 55 DEG C increasing the temperature to 80 DEG C, keeping the temperature for 3h, collecting first extraction solution, and precipitating adding the same volume of water in first precipitate, carrying out ultrasonic extraction for 30min by using ultrasonic waves under the power of 400W, and then collecting second extraction solution and residue cleaning the residue, filtering liquid which cleans the residue and obtaining third extraction solution merging the first extraction solution, the second extraction solution and the third extraction solution carrying out vacuum concentration on the extraction solution after merging to 1/6-1/10 of the total volume and adding anhydrous ethanol in the extraction solution after the vacuum concentration, leading the concentration of volume percent of the ethanol in the solution to be 60-80%, carrying out ethanol deposition and obtaining pholiota nameko polysaccharides and further purifying the pholiota nameko polysaccharides.