MnP isoenzymes produced by two Pleurotus species in liquid culture and during wheat-straw solid-state fermentation

From the paper

Abstract

Pleurotus eryngii and P. pulmonarius are being investigated for straw biopulping. They produce up to seven MnP isoenzymes which have been purified and characterized from solid-state fermentation (SSF) and liquid cultures. Pleurotus pulmonarius produces under both conditions a unique MnP, with similar pI but different molecular mass. However, P. eryngii secretes five MnP isoenzymes: three in SSF, differing in both molecular mass and pI, and two in liquid culture, differing in pI. The isoenzymes from each fungus differ in their N-terminal sequences. A noteworthy characteristic of six of them is their Mn-independent activity on 2,6-dimethoxyphenol and veratryl alcohol. Among them, those produced under liquid or SSF conditions share several characteristics, including two N-terminal consensus sequences. Finally, the isoenzyme with the highest pI, among those purified from P. eryngii SSF, is more related to Phanerochaete chrysosporium MnP.