Enhancing protein extraction from Pleurotus ostreatus using synergistic pH-shifting and ultrasonic technology: Optimization via RSM and 1H NMR-based metabolomic profiling

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Abstract

This study explores the impact of ultrasound and pH-shifting on protein extraction from P. ostreatus, employing ¹H NMR techniques to monitor metabolites and elucidate the extraction mechanism. The NMR-based metabolic analysis demonstrated that extended sonication time significantly boosted amino acid release. To optimize conditions for maximizing protein yield, response surface methodology was performed. This research aims to enhance protein extraction from P. ostreatus by analyzing the interplay between ultrasound treatment and pH-shifting. The results indicate that the synergistic use of pH-shifting and ultrasound-assisted extraction (UAE) significantly improves protein yield. The amino acid profile of the protein concentrates revealed a high content of essential amino acids, with tryptophan identified as the limiting amino acid. Additionally, the combined extraction method enhanced the emulsifying properties and foaming stability of the protein concentrates. Thus, combining pH-shifting and UAE is a promising technique for extracting high-quality protein from P. ostreatus, enhancing functional properties and efficiency.