METHOD FOR IMPROVING PRODUCTION YIELD OF ERYTHROPOIETIN FROM CHINESE HAMSTER OVARY(CHO) BY ADDING NATURAL EXTRACTS IN SERUM FREE MEDIUM, WHILE REDUCING PRODUCTION COST

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Abstract

PURPOSE: A method for improving the production yield of erythropoietin from Chinese Hamster Ovary(CHO) by adding natural extracts is provided, thereby significantly improving the production yield of CHO in compared to using a simple serum free medium, and reducing the production costs.

CONSTITUTION: The method for improving the production yield of erythropoietin from Chinese Hamster Ovary(CHO) comprises adding the extracts of one or more kinds of natural medicinal herbs into the serum free medium CHO-s SFM II, and culturing CHO in the serum free medium, wherein the natural medicinal herb is selected from Agaricus campestris var. bisporus(mushroom), Flammulina velutipes, kale, Welsh onion, tomato, Citrus unshiu MARKOVICH., Sesamum indicum L., Polygonatum stenophyllum MAX., Rehmanniae Radix Preparata, Alpinia oxyphylla MIQ., Dioscoreae Radix, onion, Liriope platyphylla WANG., Trigonella foenum-graecum L., carrot, Scrophularia buergeriana MIQ., Atractylodes macrocephala KOIDZ., Lycium Chinense, Pini Folium, Pleurotus eryngii, Cinnamomum cassia PRESL., Lentinus edodes, Morus alba Linne, Morinda offcinalis How, Cervs nippon, Schizandra chinensis BAILL., Psoralea corylifolia L., Glycyrrizae Radix, Asparagus cochinchinensis MERR., Cistanche deserticola Y. C. MA, Eucommia ulmoides OLIV., Cnidium officinale, Lilium lancifolium THUNB., Cyanachum wilfordii, Paeoniae Radix and Cornus officinalis SIEB.