Mesima, Sanghwang · 2001 · Patent
High relevanceMICROORGANISM PHELLINUS LINTEUS HAVING ANTIOXIDATION ACTIVITY
Phellinus linteus
Key points
- PURPOSE: A microorganism Phellinus linteus having an antioxidation activity is provided, which can be used as probiotics, beverage or food additives or adjuvants
- CONSTITUTION: The microorganism, Phellinus linteus ANKS9901 (KCTC 8983P), having an antioxidation activity such as superoxide dismutase(SOD), peroxidase(POD) and catalase, is isolated and identified by the steps of: sampling Phellinus linteus from the soil, examining its microbiological properties, and depositing it; isolating DNA of Phellinus linteus mycelium incubated in PDB medium by using benzylchloride method;amplifying ITS 1, 5.8S rDNA and ITS 2 genes by PCR using the isolated DNA of Phellinus linteus mycelium and determining their nucleotide sequences by using Perkin-Elmer applied biosystem ABI 377A sequencer and analyzing the correlation of Phellinus linteus with other Phellinus strains by mutialignment of Phellinus linteus ITS gene
From the paper
Abstract
PURPOSE: A microorganism Phellinus linteus having an antioxidation activity is provided, which can be used as probiotics, beverage or food additives or adjuvants.
CONSTITUTION: The microorganism, Phellinus linteus ANKS9901 (KCTC 8983P), having an antioxidation activity such as superoxide dismutase(SOD), peroxidase(POD) and catalase, is isolated and identified by the steps of: sampling Phellinus linteus from the soil, examining its microbiological properties, and depositing it; isolating DNA of Phellinus linteus mycelium incubated in PDB medium by using benzylchloride method;amplifying ITS 1, 5.8S rDNA and ITS 2 genes by PCR using the isolated DNA of Phellinus linteus mycelium and determining their nucleotide sequences by using Perkin-Elmer applied biosystem ABI 377A sequencer and analyzing the correlation of Phellinus linteus with other Phellinus strains by mutialignment of Phellinus linteus ITS gene.
Citation
CHO UNG SU, HONG SEONG DAE, HWANG GI SEOP, JUNG HYEONG JIN, KIM CHANG SU, KIM GI HUN, et al. (2001). MICROORGANISM PHELLINUS LINTEUS HAVING ANTIOXIDATION ACTIVITY.
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