King Oyster, Eryngii · 2007 · Patent
Medium relevanceBREEDING METHOD OF MUTANT OF KING OYSTER MUSHROOM
Pleurotus eryngii
Key points
- A breeding method of mutant of king oyster mushroom is provided to improve quality of king oyster mushroom by enhancing the taste and flavor, and reducing the peculiar taste of king oyster mushroom through the mutation treatment
- A breeding method of a king oyster mushroom mutant comprises the steps of: (1) putting the king oyster mushroom mycelium in a cell wall-solubilizing solution containing chitinase and a solubilizing enzyme and filtering the solution with an injector having sterilized cotton wool to separate a protoplast of king oyster mushroom mycelium (2) treating the protoplast with ultra violet(UV) having the wavelength of 35-45W as a mutation-inducing agent at a distance of 15-25 cm from the separated protoplast for 25-40 seconds and (3) regenerating the protoplasts treated by UV
From the paper
Abstract
A breeding method of mutant of king oyster mushroom is provided to improve quality of king oyster mushroom by enhancing the taste and flavor, and reducing the peculiar taste of king oyster mushroom through the mutation treatment. A breeding method of a king oyster mushroom mutant comprises the steps of: (1) putting the king oyster mushroom mycelium in a cell wall-solubilizing solution containing chitinase and a solubilizing enzyme and filtering the solution with an injector having sterilized cotton wool to separate a protoplast of king oyster mushroom mycelium (2) treating the protoplast with ultra violet(UV) having the wavelength of 35-45W as a mutation-inducing agent at a distance of 15-25 cm from the separated protoplast for 25-40 seconds and (3) regenerating the protoplasts treated by UV.
Citation
HWANG CHEOL HO (2007). BREEDING METHOD OF MUTANT OF KING OYSTER MUSHROOM.
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