ANTIOXIDANT COMPOSITION CONTAINING HISPIDIN DERIVATIVES FROM THE CULTURE BROTH OF FUNGI PHELLINUS AND INONOTUS SPP

From the paper

Abstract

A composition containing hispidin derivatives isolated from the culture broth of fungi Phellinus and Inonotus spp. is provided to scavenge superoxide radical, ABTS(2,2'-azinobis-(3-ethylbenzothiazoline-6-sulphonic acid)) radical and DPPH(1,1-Diphenyl-2-Picrylhydrazyl) radical, so that the composition is useful for preventing and treating cancer, arteriosclerosis, arthritis, cerebral apoplexy, Parkinson's disease, Alzheimer's disease and autoimmune disease. An antioxidant composition contains hispidin derivatives represented by the formula(4), formula(5) or formula(6), isolated from the culture broth of fungi Phellinus linteus KCTC 10975BP and Inonotus xeranticus KCTC 10976BP by culturing fruit body of Phellinus linteus KCTC 10975BP and Inonotus xeranticus KCTC 10976BP in potato- dextrose containing medium at 25-30 deg. C for 7-20 days, extracting the cultured broth with C1-C6 aliphatic alcohol or C5-C10 aliphatic hydrocarbon, and subjecting the extract to sephadex LH-20 column chromatography and RP-TLC(reverse phase thin layer chromatography) or preparative HPLC(high performance liquid chromatography).