PROCESS FOR CULTIVATING CROPS CONTAINING PHYTO-SELENIUM WITH MIXED MUSHROOMS

From the paper

Abstract

A method for cultivating crops containing organic selenium by using mixed mushroom germs is provided to obtain higher value-added crops containing a high concentration of organic selenium by inoculating the mixed mushroom germs into grains mixed with an inorganic selenium solution. A method for cultivating crops containing organic selenium by using mixed mushroom germs comprises the steps of: (a) adding malt to refined water, saccharifying at 55~66 DEG C for 1day, boiling at 95~110 DEG C for 20~30min, filtering, adjusting the sugar content of the malt extract to 10~13 brix, and sterilizing to make a malt extract-liquid culture medium (b) pure-culturing Phellinus linteus germs, Inonotus Obliquus germs and king oyster mushroom germs in the liquid culture medium at 15~37 DEG C for 7~14days to obtain each liquid spawn (c) putting washed grains in a culture container, and sterilizing (d) filter-sterilizing an inorganic selenium solution by a filter(0.15~0.3mum) (e) adding 80~120ppm of the filter-sterilized inorganic selenium solution to the sterilized grains to make a mixed culture medium (f) inoculating 10~15% of each liquid spawn into the mixed culture medium (g) culturing the inoculated spawns at 25~28 DEG C for 20~30days (h) adding 100ppm of an inorganic selenium solution to the mixed culture medium 2 times in the step (g) (i) shaking the culture container once every 2~3days when hyphae begin to grow on the whole surface of the grains, thereby preventing the grains from clumping together and (j) lyophilizing or hot-air drying at 65~85 DEG C, and then selecting.