Influences of Antroquinonol and 4-Acetylantroquinonol B on Inflammatory Tumorigenesis in the MCF-7 Breast Cancer Cell Line (P06-039-19)

From the paper

Abstract

Abstract Objectives The inflammatory microenvironment is currently received substantial attentions in the prevention and treatment of breast cancer (BC), the leading cause of cancer death, ∼15%, among women worldwide. The present study investigated the anti-inflammation and anti-cancer properties of 2 bioactive components from Antrodia Camphorata, a rare medicinal mushroom natively grown in Taiwan and commonly used in Chinese traditional medicine. Methods In the MCF BC cell lines with or without TNF-α stimulation, influences of Antroquinonol (AQ) and 4-Acetylantroguinonol B (4AAQB) on inflammatory mediators, aromatase, immune checkpoint and Wnt-signaling downstream genes were examined by quantitative real-time PCR. Results Among 9 inflammatory mediators (IL6, IL10, IL1β, IFNγ, PTGS2, TGFβ1, TNF-α, CCL2 andCSF1) examined, AQ inhibited 2 of them (IL-10 and PTGS2), while 4-AAQB inhibited 3 of them (IL-10, PTGS2 and TNF-α) (p Conclusions Overall, our results suggested that AQ and 4-AAQB possess the function to modulate the expression of factors related to inflammatory tumorigenesis in MCF-7 cells. Comparing these two compounds, 4-AAQB holds a more powerful modulatory effect on the expression of inflammatory mediators, aromatase as well as CD47, whereas AQ may be a more effective inhibitory compound for Wnt-signaling responses. Funding Sources This project was supported by USDA/Hatch (MAS00514) and industrial funds.