ETHANOL EXTRACT OF ANTRODIA CAMPHORATA FOR INDUCING APOPTOSIS AND PREPARATION METHOD THEREOF

Antrodia camphorata

Oncology

SpeciesNiu-Chang-Chih

JournalNot listed

Year2010

Key points

A preparation method for an ethanol extract of the fruiting body of Antrodia camphorata (EEAC) is provided
The preparation method includes steps of: (a) providing the fruiting body of A. camphorata (AC) (b) extracting the fruiting bodies with a first ethanol solution and (c) obtaining EEAC. EEAC further can be sequentially extracted or fractioned by n-hexane, ethyl acetate and ethanol, and an n-hexane fraction (FC), an ethyl acetate fraction (FA) and an ethanol fraction (FB) respectively are generated
The growth inhibition and apoptosis induction of leukemia cell line HL 60 are effectively mediated by FA product, in which zhankuic acid A is the bioactive marker
The amount of triterpenoid in the fruiting body of AC can be determined by NMR and HPLC analysis

From the paper

Abstract

A preparation method for an ethanol extract of the fruiting body of Antrodia camphorata (EEAC) is provided. The preparation method includes steps of: (a) providing the fruiting body of A. camphorata (AC) (b) extracting the fruiting bodies with a first ethanol solution and (c) obtaining EEAC. EEAC further can be sequentially extracted or fractioned by n-hexane, ethyl acetate and ethanol, and an n-hexane fraction (FC), an ethyl acetate fraction (FA) and an ethanol fraction (FB) respectively are generated. The growth inhibition and apoptosis induction of leukemia cell line HL 60 are effectively mediated by FA product, in which zhankuic acid A is the bioactive marker. The amount of triterpenoid in the fruiting body of AC can be determined by NMR and HPLC analysis.

Citation

WU YANG-CHANG, LU MEI-CHIN, CHANG FANG-RONG, DU YING-CHI, WU TUNG-YING (2010). ETHANOL EXTRACT OF ANTRODIA CAMPHORATA FOR INDUCING APOPTOSIS AND PREPARATION METHOD THEREOF.

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