ANTI-CANCER ACTIVE SUBSTANCE FROM ANTRODIA CAMPHORATA, METHOD FOR PREPARING THE SAME AND USE THEREOF

From the paper

Abstract

An anti-cancer active substance from Antrodia Camphorata, produced from mycelium of Antrodia Camphorata by following steps: (1) culturing mycelium of Antrodia Camphorata by liquid fermentation (2) extracting the thus-obtained mycelium with ethanol and concentrating the extracted solution to obtain an ethanol extract (3) dissolving the ethanol extract in water, and extracting with equal volume of ethyl acetate to obtain ethyl acetate extract (4) purifying the ethyl acetate extract by silica gel column chromatography using hexane/ethyl acetate gradient as the mobile phase to obtain an eluate fraction g and (5) purifying the eluate fraction g by silica gel column chromatography using hexane/ethyl acetate gradient as the mobile phase to obtain a specific eluate fraction which could be purified further to obtain the anti-cancer active substance from Antrodia Camphorata.