Zhu Ling, Umbrella Polypore · 2004 · English Abstract
Medium relevance[The effect of polyporus umbellatus polysaccharide on the immunosuppression property of culture supernatant of S180 cells].
Polyporus umbellatus
Key points
- AIM: To investigate the effect of polyporus umbellatus polysaccharide (PUPS) on the immunosuppression property of tumor cell line S180 culture supernatant
- METHODS: The inhibitory effects of the culture supernatant of S180 cells in the presence or absence of PUPS on the ConA-induced mouse splenocyte proliferation, IL-2 production, killer activity and the reactivity of CTLL-2 cells to IL-2 were detected by MTT colorimetry
- The effect of the culture supernatant on the IL-2Ralpha expression on murine splenocytes was detected by flow cytometry
- RESULTS: The culture supernatant of S180 cells could strongly inhibit immunity in terms of the above five indexes, while PUPS could reverse the immunosuppression
- CONCLUSION: PUPS can offset the immunosuppression of the supernatant from S180 cell culture, which may be mediated by down-regulating the synthesis and/or secretion of immunosuppressive substance by S180 cells
Metadata-grounded summary
Citation abstract
AIM: To investigate the effect of polyporus umbellatus polysaccharide (PUPS) on the immunosuppression property of tumor cell line S180 culture supernatant.
METHODS: The inhibitory effects of the culture supernatant of S180 cells in the presence or absence of PUPS on the ConA-induced mouse splenocyte proliferation, IL-2 production, killer activity and the reactivity of CTLL-2 cells to IL-2 were detected by MTT colorimetry. The effect of the culture supernatant on the IL-2Ralpha expression on murine splenocytes was detected by flow cytometry.
RESULTS: The culture supernatant of S180 cells could strongly inhibit immunity in terms of the above five indexes, while PUPS could reverse the immunosuppression.
CONCLUSION: PUPS can offset the immunosuppression of the supernatant from S180 cell culture, which may be mediated by down-regulating the synthesis and/or secretion of immunosuppressive substance by S180 cells.
Citation
Yang LJ, Wang RT, Liu JS, Tong H, Deng YQ, Li QH (2004). [The effect of polyporus umbellatus polysaccharide on the immunosuppression property of culture supernatant of S180 cells]. Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology PMID: 15191734
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