Enoki, Winter Mushroom · 2003 · English Abstract
Low relevance[A simple and effective method for extracting total RNA from domestic fungus].
Flammulina velutipes
Immune support
Key points
- With Flammulina velutipes material,an improved method was developed for extracting total RNA from domestic fungus that are rich in RNase,polyphenols, polymeric carbohydrates and proteoglycans
- Phenol-chloroform-isoamyl alcohol were used twice to clear DNA and protein under higher concentration of denaturing solution and isopentanol, sodium acetate were used to precipitate RNA selectively
- Pure and intact RNA can be effectively prepared by this method
Metadata-grounded summary
Citation abstract
With Flammulina velutipes material,an improved method was developed for extracting total RNA from domestic fungus that are rich in RNase,polyphenols, polymeric carbohydrates and proteoglycans. Phenol-chloroform-isoamyl alcohol were used twice to clear DNA and protein under higher concentration of denaturing solution and isopentanol, sodium acetate were used to precipitate RNA selectively. Pure and intact RNA can be effectively prepared by this method.
Citation
Zhou KS, Xue JG, Chang N, Chen C, Zhang HX, Zhang CK (2003). [A simple and effective method for extracting total RNA from domestic fungus]. Yi chuan = Hereditas PMID: 15639964
Open citation